Effect of the combination of hyperbaric oxygen and 5-fluorouracil on the proliferation and metastasis of nasopharyngeal carcinoma CNE-2Z cell line.
- Author:
Zhengrong PENG
1
;
Weihong ZHONG
;
Juan LIU
;
Pingtian XIAO
Author Information
1. Department of Hyperbaric Oxygen, Xiangya Hospital of Central South University, Changsha, 410008, China. pengzr138@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Fluorouracil;
therapeutic use;
Humans;
Hyperbaric Oxygenation;
Matrix Metalloproteinase 9;
metabolism;
Nasopharyngeal Neoplasms;
pathology;
therapy;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(9):386-391
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and the possible influential mechanisms of hyperbaric oxygen (HBO) and 5-fluorouracil (5-FU) on the proliferation and metastasis in human nasopharyngeal carcinoma (NPC) CNE2Z cell line.
METHOD:NPC CNE2Z cell line were divided into 4 groups randomly: group A: control group; group B: 5-FU group; group C: HBO group; group D: 5-FU and HBO group. The inhibition effect of proliferation in CNE2Z cells of all groups through 24 h, 48 h and 72 h disposal was detected by MTT reduction assay; Transwell chamber assay was performed to determine the effect of HBO and (or) 5-FU on the metastasis of the CNE2Z cells; The MMP-9, VEGF expression in CNE2Z cells of all groups were detected by SP immunocytochemical stain and observe the expressed image by micro.
RESULT:There were statistical difference on the inhibition effect of proliferation in C group and A group after 48 h and 72 h disposal (P<0.01) and between A, B, C group and D group only after 48 h disposal (P<0.05); There were not statistical difference on the effect of metastasis in C group and A group (P>0.05) and between B, C group and D group (P>0.05); Average optical of the MMP-9, VEGF expression were not statistically significant difference in C group and A group (P>0.05) and between C group and D group (P>0.05).
CONCLUSION:Simple HBO disposal after 48 h and 72 h could inhibit the proliferation potential of NPC CNE2Z cell line, but the combination of HBO and 5-FU only after 48 h disposal could all the more inhibit the proliferation potential. Simple HBO disposal couldn't decrease the MMP-9 and VEGF high-expression and inhibit the metastasis potential in human NPC CNE2Z cell line, the combination of HBO and 5-FU disposal also couldn't further decreased the MMP-9 and VEGF high-expression and inhibited the metastasis potential.
