Expression and significance of integrins subunits in laryngeal squamous cell carcinoma.
- Author:
Rongsheng NI
1
;
Xiaohui SHEN
;
Haiyan WU
;
Wenyan ZHU
;
Jie NI
;
Zhenghua HUANG
;
Yongling SONG
;
Xia GAO
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Nanjing BenQ Hospital (the Affiliated Hospital of Nanjing Medical University), Nanjing, 210008, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Aged, 80 and over;
Carcinoma, Squamous Cell;
metabolism;
pathology;
Humans;
Integrin alpha Chains;
genetics;
metabolism;
Integrin alphaV;
genetics;
metabolism;
Integrin beta Chains;
genetics;
metabolism;
Laryngeal Neoplasms;
metabolism;
pathology;
Male;
Middle Aged;
Neoplasm Proteins;
metabolism;
Neoplasm Staging
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(15):686-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study was to investigate the expression and significance of Integrins subunits in laryngeal squamous cell carcinoma (LSCC).
METHOD:The expression of Integrins subunits was detected by cDNA microarray in 4 cases of primary LSCC tissues and corresponding adjacent normal tissues. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to identify the different expression of Integrins subunits in 24 cases of primary LSCC tissues and corresponding adjacent normal tissues.
RESULT:A cDNA microarray analysis revealed significant changes in the expression of Integrins subunits, with IntegrinalphaV, Integrinbeta8 being up-regulated and Integrinalpha8 being down-regulated. The result of RT-PCR was consistent with that of cDNA microarray. The mRNA levels of IntegrinalphaV and Integrinbeta8 were significantly higher in LSCC tissues than that in corresponding adjacent normal tissues (1.0131 +/- 0.4780 vs 0.7591 +/- 0.4678 for IntegrinalphaV, P<0.05, 1.7362 +/- 1.3849 vs 1.2267 +/- 0.9363 for Integrinbeta8, P<0.05). The mRNA levels of Integrinalpha8 were significantly lower in LSCC tissues than that in corresponding adjacent normal tissues (0.2646 +/- 0.2622 vs 0.5457 +/- 0.3827, P<0.05).
CONCLUSION:The expression of IntegrinalphaV, Integrinbeta8, Integrinalpha8 were significantly up-regulated or down-regulated in laryngeal squamous cell carcinoma, which may relate to tumorigenesis and development of laryngeal squamous cell carcinoma.
