Autoradiography observation on cochlea and organs in guinea pigs after intra-abdominal injection of bFGF.
- Author:
Suoqiang ZHAI
1
;
Panzao CHEN
;
Wei GUO
;
Ning YU
;
Jainhe SUN
;
Yinyan HU
Author Information
1. Institute of Otolaryngology Head and Neck Surgery of PLA General Hospital, Beijing, 100853, China. zhaisq@plagh.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Autoradiography;
Cochlea;
cytology;
metabolism;
Fibroblast Growth Factor 2;
administration & dosage;
Guinea Pigs;
Injections, Intraperitoneal;
Iodine Radioisotopes;
administration & dosage
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(16):750-752
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe whether bFGF could cross the blood-labyrinth barrier (BLB) after intra-abdominal injection and to establish an experimental basis for its clinical applications.
METHOD:Thirty guinea pigs were divided into three groups. Animals in group 1 were administered o I-bFGF, while animals in group 2 and 3 were administered 125 and saline, respectively, via intra-abdominal injection. The both cochlea, blood, liver, brain, thyroid gland and kidney were collected and weighted. A radioimmunoassay analyzer was employed to measure counts per minute (CPM) of each sample, and autoradiography was performed on both cochlea.
RESULT:The CPM value of organ samples in the 125I group was higher than that in other groups, and radioactive grain was observed in cochlear samples of this group. In the 125I-bFGF group, blood demonstrated the highest CPM value, while cochlea and brain demonstrated the lowest CPM value, with no radioactive grain observed in cochlear samples.
CONCLUSION:bFGF has some difficulties in getting across BLB, so the way of bFGF application in clinics need further study.
