Studies on the Effects of Monoclonal Antibodies to Mammalian Heat Shock Protein 60 (HSP 60) on Mouse Embryo Development In Vitro.
- Author:
Il Han LEE
1
;
Kyung Nam CHUNG
;
Yong Bong KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Inje University, Seoul Paik Hospital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Heat shock protein;
Monoclonal antibody;
Embryo development
- MeSH:
Animals;
Antibodies;
Antibodies, Monoclonal*;
Apoptosis;
Chaperonin 60*;
Embryonic Development*;
Embryonic Structures*;
Female;
Heat-Shock Proteins*;
Hot Temperature*;
Humans;
Immunoglobulin G;
Mice*;
Mice, Inbred ICR;
Pregnancy
- From:Korean Journal of Obstetrics and Gynecology
2003;46(11):2216-2220
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the effects of antibodies to HSP 60 on the early mouse embryo development in vitro. METHODS: The 175 late 2-cell mouse embryos were obtained from 6-7 week old female ICR mice. 5-10 embryos were placed in each well. The embryos were incubated in the Ham's F-10 medium supplemented with 100 microgram/mL of monoclonal antibody to HSP 60 (66), monoclonal mouse IgG1 (55), and medium alone (54), respectively, at 37degrees C in a 5% CO2 humidified air chamber, and mouse embryo developments were observed daily. RESULTS: On day4, growth arrests were more prominent in anti-HSP 60 containing group compared to IgG1 containing group, medium only group (0% vs 16%, 14%), and these results were statistically significant (p=0.0032). Especially those inhibitory effects were observed in early stage of embryo development (day1) and these results were also statistically significant (31% vs 83%, 77%, p<0.0001). Moreover, we found out that cellular degenerations were more common in anti-HSP 60 containing group and this features were prominent on day2. CONCLUSION: Anti-HSP 60 elicited a strong growth inhibitory and degenerative effect on early mouse embryo development. These findings suggest that HSP 60 may exert a protective effect against mouse embryo degeneration or apoptosis.
