Effect of formaldehyde inhalation on allergic rhinitis in mice.
- Author:
Rong XIANG
;
Yu XU
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, Dermatophagoides;
Arthropod Proteins;
Cysteine Endopeptidases;
Disease Models, Animal;
Enzyme-Linked Immunosorbent Assay;
Formaldehyde;
adverse effects;
Inflammation;
Inhalation Exposure;
adverse effects;
Interferon-gamma;
blood;
Interleukin-10;
blood;
Interleukin-4;
blood;
Male;
Mice;
Mice, Inbred BALB C;
Nasal Mucosa;
Rhinitis, Allergic;
chemically induced
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(16):1467-1471
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of formaldehyde inhalation on the allergic rhinitis mice model.
METHOD:Forty-eight male BALB/C mice in six experimental group were exposure to (A) saline control; (B) Der p1; (C) formaldehyde (3.0 mg/m3); (D) Derp1 + formaldehyde (1.5 mg/m3); (E) Der p1 + formaldehyde (3.0 mg/M3); (F) Der p1+ formaldehyde (6.0 mg/m3). The concentrations of IL-4, IL-10 and IFN-γ in the peripheral serum were measured by enzyme-linked immunosorbent assay(ELISA). Nasal mucosal inflammation was evaluated by HE staining. Result: Formaldehyde exposure could increase the number of allergic rhinitis mice with sneezing and rubbing nose. The levels of IL-4 and IL-10 in group B, D, E and F were higher than that ingroup A (P < 0.05). Compared with the group C, the group D, E and F could effectively increase serum IL-4 and IL-10. The concentration of IL-4 in group E and F was higher than that of group B, while the group C was lower (P < 0.05). The concentration of IL-10 in group D, E and F was higher than that in group B (P < 0.05). The expression of IFN-γ in group B, D, E and F was lower than that in group A. While, the IFN-γ expression in group B was lower than that of group C and higher than that in group F (P < 0.05). Moreover, the concentration of IFN-γ in group D, E and F was lower compared with group C (P < 0.05). The nasal mucosa HE staining showed that the density of EOS increased simultaneously in formaldehyde exposure allergic rhinitis groups.
CONCLUSION:The study showed that formaldehyde exposure can promote Th2 cytokines and eosinophil infiltration and then aggravate the allergic rhinitis symptoms.
