Mutation analysis of 16 mutation spots related to children patients with non-syndromic sensorineural hearing loss.
- Author:
Rongjun MAN
;
Zeng ZHANG
;
Rongzhong LU
;
Xiao WANG
;
Shiping SUN
;
Dan WANG
;
Xiaosong XU
;
Weiguo WANG
;
Huizhong WANG
- Publication Type:Journal Article
- MeSH:
Alleles;
Child;
Connexins;
DNA Mutational Analysis;
DNA, Mitochondrial;
Deafness;
Gene Frequency;
Hearing Loss;
Hearing Loss, Sensorineural;
genetics;
Heterozygote;
Homozygote;
Humans;
Mitochondria;
Mutation;
Polymerase Chain Reaction;
RNA, Ribosomal
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(4):319-324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the clinical signification of screening 16 target deafness mutations in GJB2, GJB3, SLC26A4, WFS1 and mitochondrial DNA 12S rRNA in 135 children patients with non-syndromic sensorineural hearing loss (NSHL) in Zibo City, Shandong province.
METHOD:Peripheral blood samples of 135 subjects in the study diagnosed as NSHL were collected; Polymerase chain reaction (PCR) and direct sequencing were used to analyze the 16 mutation spots.
RESULT:Sixty-two cases of 135 patients (45.9%, 62/135) were found out to be carries of at least one pathogenic gene mutation. Among them, 24 cases (17.8%, 24/135) had two mutated alleles (homozygote and compound heterozygote), and 38 cases (28.1%, 38/135) were single mutant carriers. Among all the children patients, 30 cases (22. 2%, 30/135) had SLC26A4 mutations, and 19 cases (14.1%, 19/135) had GJB2 mutations. In the study 86 Mutant alleles were detected, and the allele frequency of SLC26A4 c. 766_2A > G and GJB2 c. 235delC was 11.11% (30/270) and 8.5% (23/270) respectively. The allele frequency of SLC26A4 c. 2168A > G and WFS1 c. 2158A > G is 2.6% (7/270).
CONCLUSION:SLC26A4 mutation is the primary cause of the patients with NSHL in this study, and GJB2 mutation is the secondary. The most common mutant form is c. 766_2A of SLC26A4, and the second is c. 235delC of GJB2. GJB3 and WFS1 mutations were detected, whereas mtDNA mutations were not found out in this study.
