Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells.
- Author:
Ping XIAO
;
Quiyang FU
;
Zeyong LI
;
Xuehui ZHANG
;
Xiangjun FU
;
Renqiang WAN
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Carcinoma, Squamous Cell;
metabolism;
pathology;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Guanine Nucleotide Exchange Factors;
genetics;
metabolism;
Head and Neck Neoplasms;
metabolism;
pathology;
Humans;
Squamous Cell Carcinoma of Head and Neck;
T-Lymphoma Invasion and Metastasis-inducing Protein 1;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(7):633-637
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.
METHOD:Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.
RESULT:Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05).
CONCLUSION:Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
