The relation between age and expression of Myosin VI and Disabled-2 in the cochlea of mice.
- Author:
Zhiji CHEN
;
Xiang GU
;
Ting CAI
;
Rui SONG
;
Xiaoqing ZHOU
;
Wei YUAN
- Publication Type:Journal Article
- MeSH:
Adaptor Proteins, Vesicular Transport;
metabolism;
Aging;
Animals;
Cochlea;
metabolism;
Endocytosis;
Hair Cells, Auditory;
metabolism;
Hair Cells, Auditory, Inner;
metabolism;
Mice;
Microscopy, Confocal;
Myosin Heavy Chains;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(7):645-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of Myosin VI and Disabled-2 (Dab2) in the cochlea of mice at different ages.
METHOD:Forty KM mice were divided into four groups according to age, named as postnatal 2 week (P2w), P5w, P9w, P16month. The localization of protein in the basilar membrane of mice cochlea was detected by immunofluorescence staining and laser scanning confocal microscope (LSCM). The mRNA expression level of protein in cochlear at different ages was evaluated by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Statistical analysis was performed by the SPSS18.0 software.
RESULT:Myosin VI and Disabled-2 protein mainly expressed at the apical cytoplasm of hair cells. As for the inner hair cell, Dab2 labeling was abundant especially at the cuticular plate and nearby. Comparing four immunofluorescence staining images of Myosin VI, we found the fluorescence intensity of P2w and P16m were weaker than that of P5w and P9w. After setting P9w as the control group, qRT-PCR revealed that the mRNA expression of MyosinVI and Dab2 in P2w was less than that in the control group (P < 0.01), while no significant difference was found between P5w and the control group, nor between P16m and the control group (P > 0.05).
CONCLUSION:Myosin VI and Dab2, two proteins which regulated the clathrin-mediated endocytosis, expressed at hair cells of mice cochlea. In the inner hair cell, this process of endocytosis may be more efficient at the cuticular plate and nearby. The expression level of protein may change in different ages, and this probably leads to a difference of CME, it also may cause a defect of inner hair cells function.
