Different methods of antigen retrieval impact on Pan of sinonasal inverted papilloma.
- Author:
Bo YU
;
Chen LI
;
Hui WANG
;
Ying GE
;
Hui KONG
;
Li KONG
- Publication Type:Journal Article
- MeSH:
Antigens;
analysis;
Buffers;
Humans;
Immunohistochemistry;
methods;
Microwaves;
Nose Neoplasms;
immunology;
Papilloma, Inverted;
immunology;
Paranasal Sinus Neoplasms;
immunology;
Staining and Labeling;
methods
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(17):1311-1313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore different conditions and buffers of antigen retrieval which affect the CK of SNIP on immunohistochemical staining results.
METHOD:Dividing paraffin tissue sections of 11 patients into four groups. Using the Image-Pro Plus Image analyzer and taking five horizons for each section to calculate an average of 200 areas, measured standard optical density of the positive reaction areas.
RESULT:It is divided into four groups: high temperature and high pressure citrate buffer retrieval, microwave EDTA buffer retrieval, microwave citrate retrieval, high temperature and high pressure EDTA buffer retrieval. The standard optical density of positive reaction areas respectively express: 0.324 ± 0.051, 0.325 ± 0.056, 0.303 ± 0.061, 0.365 ± 0.023. The rates of CK positive expression with high temperature and high pressure EDTA buffer retrieval is batter than other repairing groups in the same paraffin tissue sections (P < 0.05).
CONCLUSION:For the Pan of Sinonasal inverted papilloma, the method of high temperature and high pressure EDTA buffer antigen retrieval can achieve the ideal staining results? which is worth while to promote and maybe as a bet? ter guide of clinic work.
