Inhibitive effect of matrine modification X on the growth of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice.
- Author:
Shujing SHI
;
Anzhou TANG
;
Shaolin YIN
;
Lisheng WANG
;
Mao XIE
;
Xiang YI
- Publication Type:Journal Article
- MeSH:
Alkaloids;
pharmacology;
Animals;
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
Carcinoma;
Caspase 3;
metabolism;
Cell Line, Tumor;
Heterografts;
Humans;
Mice;
Mice, Nude;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Quinolizines;
pharmacology;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(21):1697-1700
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the inhibitive effect of matrine modification X on the growth of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice.
METHOD:Tumor model was established by subcutaneous inoculation of nasopharyngeal carcinoma cell CNE2 into nude mice, which was used to evaluate the antitumor effect of matrine modification X in vivo. The expression levels of Bax, Bcl-2, Caspase3 were detected by real-time PCR and western blot.
RESULT:The growth of xenografts in nude mice was significantly suppressed after application of matrine modification X in a dose-dependent manner. The inhibition rates were 32.55% and 44.89% when treated at medium and high dose respectively. Real-time fluorescence quantitative-PCR and Western Blot results showed that the expression of Bax and Caspase3 increased, while the expression of Bcl-2 decreased in a dose-dependent manner. The change of high dose group was obvious, and the difference was statistically significant (P < 0.05).
CONCLUSION:Matrine modification X could significantly inhibit the growth of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice, probably by inducing the apoptosis of nasopharyngeal carcinoma cells, and the possible mechanism is related to regulating the expression level of Bax/Bcl-2 and Casepase3.
