The expression levels of three T-type calcium channel receptors in inner ear of C57BL/6J mice with age.
- Author:
Chen PAN
;
Yafeng YU
;
Hongyang LING
;
Wenying WU
;
Gensheng XIAO
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium Channels;
Calcium Channels, T-Type;
biosynthesis;
Cochlea;
metabolism;
Ear, Inner;
Mice;
Mice, Inbred C57BL;
Neurons;
Receptors, Calcium-Sensing;
Spiral Ganglion;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(22):1791-1794
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression levels of three T-type calcium channel receptors (α1G; α1H; α1I) in the cochlea and spiral ganglion neurons of C57BL/6J mice with different ages.
METHOD:Thirty cases of C57BL/6J mice were divided into three groups (6-8 W, 24-26 W, 42-44 W) according to the age. The expressions of three T-type calcium channel receptors were quantified by RT-PCR after hearing thresholds measured by ABR.
RESULT:Three receptors were detected in the cochlea and spiral ganglion neurons of 6-8 W C57BL/6J mice. The quantitative results showed that the expression levels of α1H and α1I were highest among three receptors in spiral ganglion neurons and in the cochlea respectively. The expression levels of three receptors significantly decreased with age,especially at the age of 4244 W.
CONCLUSION:The expression of T-type calcium channel receptors reduced with age in the inner ear of C57BL/6J mice.
