Evaluation of Latex Agglutination Test Kit for Rapid Detection of Methicillin-Resistance in Staphylococcus aureus.
- Author:
Yeon Joon PARK
1
;
Eun Jee OH
;
Jeong Sook YOUN
;
Seung Ok LEE
;
Young Joo NA
;
Jeong Joon PARK
;
Byung Kee KIM
Author Information
1. Department of Clinical Pathology, College of Medicine, the Catholic University of Korea
- Publication Type:Original Article
- Keywords:
Staphylococcus aureus;
Latex agglutination;
Methicillin-resistance;
detection
- MeSH:
Agar;
Agglutination;
Diffusion;
Latex Fixation Tests*;
Latex*;
Mass Screening;
Methicillin Resistance;
Methicillin-Resistant Staphylococcus aureus;
Oxacillin;
Polymerase Chain Reaction;
Sensitivity and Specificity;
Staphylococcus aureus*;
Staphylococcus*
- From:Korean Journal of Clinical Pathology
1999;19(6):667-671
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The identification of methicillin-resistant Staphylococcus aureus (MRSA) in the clinical laboratory has been typically performed by using methods that detect phenotypic expression of resistance determinants. However, these methods are not always reliable since phenotypic expression of methicillin resistance is known to be heterogeneous. In this study, MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, agar dilution MIC and PCR of the mecA gene for the detection of methicillin resistance in S. aureus. METHODS: A total of 48 MRSA and 46 methicillin-susceptible S. aureus (MSSA) clinical isolates, defined by the presence or absence of the mecA gene, respectively, were tested. RESULTS: The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivity of 97.9%, 93.8%, and 97.9% and specificity of 100% in all three tests. CONCLUSION: We conclude that the MRSA-Screen test is very accurate, reliable and easy to perform for detection of methicillin resistance in S. aureus.
