Comparison of serotypes, restriction enzyme analysis of plasmid DNA pattern and PFGE(pulsed-field gel electrophoresis) patterns of Yersinia pseudotuberculosis isolates in Korea..
- Author:
Me Ae KIM
1
;
Chong Rae CHO
;
In Ki PAIK
;
Jong In PARK
;
Jeong Nyeo LEE
Author Information
1. Department of Clinical Pathology, Inje University College of Medicine, Sanggye Paik Hospital
- Publication Type:Original Article
- Keywords:
Yersinia pseudotuberculosis;
Restriction enzyme analysis of plasmid DNA;
PFGE
- MeSH:
Animals;
Diarrhea;
Disease Outbreaks;
DNA*;
Electrophoresis, Gel, Pulsed-Field;
Humans;
Korea*;
Mice;
Plasmids*;
Restriction Mapping*;
Seoul;
Yersinia pseudotuberculosis*;
Yersinia*
- From:Korean Journal of Clinical Pathology
1999;19(6):689-695
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Yersinia pseudotuberculosis, a member of genus Enterobactericeae, is a main etiologic organism of diarrhea in childhood. Because a mouse and a unchlorinated spring water are main reservoirs of Y. pseudotuberculosis, the strains from a contaminated spring water and mouse could be involved in human epidemic. The purpose of this study was to investigate a clonality between the strains from patients and those from an unchlorinated spring water and a mouse by restriction enzyme analysis of plasmid DNA and pulsed-field gel electrophoresis (PFGE). METHOD: We isolated 15 Y. pseudotuberculosis strains including 8 isolates from patients (S1-S8), 6 isolates from mountain water (W1-W6), 1 isolate from a mouse (M1) in northeast area of Seoul. Plasmid and chromosomal DNA of all strains were analyzed by REAP with Bam H1 restriction and by PFGE with Xba I restriction , respectively. RESULTS: Restriction enzyme analysis of plasmid DNA was classified into type B and type D. All 7 strains of serotype 15 were classified as type B and 8 strains of serotype 4b were classified as type D. PFGE were classified into 6 different types. Among them, strains of PFGE type I, II, III, IV belong to Y. pseudotuberculosis serotype 15 and Y. pseudotuberculosis 4b strains were classified into PFGE type V, VI. S1 and W1 were classified into PFGE type I . S8, W6 and M1 were classfied into PFGE type VI. CONCLUSIONS: PFGE revealed clonality among strains from patients, a water and a mouse. PFGE was more discriminative than REAP to characterize the Y. pseudotuberculosis outbreaks in Korea.
