Expression and prognostic significance of cyclooxygenase-2 in supraglottic laryngeal squamous cell carcinomas.
- Author:
Jun LI
1
;
An ZHANG
;
Xiaoqiu CHEN
;
Zhong REN
;
Zimin PAN
Author Information
1. Department of Otorhinolaryngology, Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China. lisacmu2@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Carcinoma, Squamous Cell;
diagnosis;
metabolism;
pathology;
Cyclooxygenase 2;
metabolism;
Female;
Humans;
Laryngeal Mucosa;
metabolism;
pathology;
Laryngeal Neoplasms;
diagnosis;
metabolism;
pathology;
Male;
Middle Aged;
Neoplasm Staging;
Prognosis
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(24):1109-1113
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression and prognostic significance of cyclooxygenase-2 (Cox-2) in supraglottic laryngeal squamous cell carcinomas (SGLSCC) and identify the relationship between Cox-2 and angiogenesis and the roles of Cox-2 in SGLSCC as a biological marker.
METHOD:Eighty-eight primary SGLSCC patients received surgical treatment were studied by immunohistochemical staining, and reverse transcription-polymerase chain reaction (RT-PCR) technique.
RESULT:The percentage of Cox-2-positive cells was 94.3% (83/88) in SGLSCC whereas there was no immunostaining in the all cells of normal mucosa of paracarcinoma. Cox-2 expression was higher in well-differentiated tumors compared with poorly-differentiated SGLSCC. The relative concentration of Cox-2 mRNA was 141.871 +/- 20.5435 in SGLSCC and 17.031 +/- 2.2597 in normal paracarcinoma mucosa (P < 0.01). It was significantly higher in SGLSCC than in normal paracarcinoma mucosa. In SGLSCC, only pathological grading and the percentage of Cox-2-positive cells had significant correlation (P < 0.01). And not only the percentage of Cox-2-positive cells but also Cox-2 intensity had significant correlation with microvessel density (MVD) (P < 0.01). Kaplan-Meier survival analysis showed there had significant relationship between Cox-2 intensity and cumulative survival rate of SGLSCC patients (P < 0.05). But the percentage of Cox-2-positive cells was different (P > 0.05). Cox's regression analysis indicated that Cox-2 intensity were significantly independent prognostic factors (P < 0.01).
CONCLUSION:Cox-2 expression maybe relate to the carcinogenesis and progress in tumors especially in well-differentiated ones; The changes of Cox-2 expression are synchronous with MVD. Cox-2 intensity is a significantly independent prognostic factor. So Cox-2 may be an effective target of prevention, therapy and prognostic evaluation for laryngeal carcinoma and other head and neck squamous cell carcinomas (HNSCC).
