Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells.
- Author:
Feng ZHANG
1
;
Yonghua CUI
;
Ping CAO
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. lhkzhangfeng@163.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Quercetin;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(24):1136-1139
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of quercetin (Que) on HEN1 cells, a kind of human nasopharyngeal carcinoma cells.
METHOD:Inhibition rate of quercetin on HEN1 was assayed by MTT method, cell apoptosis by flow cytometry (FCM), Caspase-3 expression of each group were determined by colorimetry set.
RESULT:The inhibition of quercetin on HEN1 cells was shown in the dose-dependent (r = 0.709, P < 0.05) and time-dependent manner (r = 0.703, P < 0.01). The ratio of apoptotic and necrosis cells increased accompanied with added quercetin concentration. Cell cycle was specificly arrested inG2/M phase. Apoptosis cusp was revealed by FCM. While the activity of caspase-3 were also significantly upregulated in five groups after quercetin compared to control (P < 0.05).
CONCLUSION:Quercetin can activate the expression of Caspase-3 and induce the apoptosis of HEN1 cells through mitochondrion-depended pathway.
