Expression and significance of nuclear factor-kappaB and ICAM-1 mRNA in nasal mucosa of allergic rhinitis.
- Author:
Jiandong ZHAO
1
;
Yanjun WANG
;
Weijia KONG
Author Information
1. Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhaojd.jack@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adult;
Case-Control Studies;
Female;
Humans;
Intercellular Adhesion Molecule-1;
metabolism;
Male;
NF-kappa B p50 Subunit;
metabolism;
Nasal Mucosa;
metabolism;
RNA, Messenger;
genetics;
Rhinitis;
metabolism;
pathology;
Transcription Factor RelA;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(2):57-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To clarify the pathogenesis of allergic rhinitis (AR) by detecting the activation of NF-kappaB and the expression of ICAM-1 mRNA in nasal mucosa of the patients with AR.
METHOD:AR patients were collected as experimental group and deflection of nasal septum patients as control group. Immunohistochemistry methods were used to examine the expression of NF-kappaB p50 and p65 subunits in the middle turbinate mucous tunic. ICAM-1 mRNA were detected by means of RT-PCR.
RESULT:NF-kappaB was strongly expressed in AR group. NF-kappaB p50 existed in cytoplasm and nucleus. NF-kappaB p65 was mainly expressed in cytoplasm and less in nucleus. While NF-kappaB was expressed little in control group. The difference of NF-kappaB p50 and NF-kappaB p65 expression between two groups were significantly (P < 0.01). ICAM-1 mRNA was more strongly expressed in AR group (P < 0.01) while feebly expressed in deflection of nasal septum group. The expression of ICAM-1 mRNA were significantly correlated with that of NF-kappaB p50 and NF-kappaB p65 (r = 0.899 5, P < 0.01; r = 0.7601, P < 0.01).
CONCLUSION:NF-kappaB plays a key role in AR. The excessively activated NF-kappaB promotes the transcription of ICAM-1 mRNA. ICAM-1 relates to the pathogenesis and development of AR.
