Analysis of EBV-LMP1 gene mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing.
- Author:
Yucheng YANG
1
;
Yiqin WANG
;
Di QIAN
;
Yun HE
;
Suling HONG
Author Information
1. Department of Otolaryngology, the First Affiliated Hospital, Chongqing University of Medical Science, Chongqing, 400016, China.
- Publication Type:Journal Article
- MeSH:
Adult;
China;
DNA, Viral;
genetics;
Female;
Herpesvirus 4, Human;
Humans;
Male;
Middle Aged;
Nasopharyngeal Neoplasms;
blood;
genetics;
pathology;
virology;
Sequence Deletion;
Viral Matrix Proteins;
blood;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(4):153-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect and analyze the EBV-LMP1 gene N terminus XhoI-site mutation and C terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (NPC) in Chongqing of China.
METHOD:DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China. All the PCR production of N terminus was digested by enzyme XhoI, then segregated in 8% PAGE. All- the PCR production of C terminus was segregated in 8% PAGE too. Compared with B95-8 cell, some of the PCR production were sequenced and analyzed with software.
RESULT:LMP1 was amplified successfully from 48 of 48 NPC cases (100%) and from 38 of 40 non-NPC cases (95%) by PCR, but none of LMP1 XhoI-site mutation and none of 30 bp deletion was found by digesting, segregating and Sequencing.
CONCLUSION:EBV-LMP1 gene N terminus XhoI-site and C terminus 30 bp deletion have no mutation in plasma of NPC or non-NPC cases in Chongqing of China. The precise relationship of EBV-LMP1 gene mutation or deletion with NPC pathogenesis needs further investigation.
