The effect of microwave radiation on the levels of MDA and the activity of SOD of nasopharyngeal carcinoma cells.
- Author:
Jing MA
1
;
Bin LIU
Author Information
1. Department of Otorhinolaryngology, Lanzhou Generation Hospital, Lanzhou Command, Lanzhou, 730050, China. majing6410@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Dose-Response Relationship, Radiation;
Gene Expression Regulation, Neoplastic;
Humans;
Malondialdehyde;
metabolism;
radiation effects;
Microwaves;
Nasopharyngeal Neoplasms;
metabolism;
Superoxide Dismutase;
metabolism;
radiation effects;
Up-Regulation
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(4):160-162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of microwave radiation on the levels of malondialdehyde (MDIA) and activity of superoxide dismutase (SOD) of Nasopharyngeal carcinoma cells.
METHOD:The experiment were divided into four groups included control, 10, 20 and 30 mW/cm2 groups. The methods, such as TBA colorimetric assay and modified NBT hydroxylamine method, were used to detect the effect of microwave radiation on the levels of MDA and activity of SOD of nasopharyngeal carcinoma cells.
RESULT:With increase of microwave radiation, TBA assay showed that the levels of MAD were 1.4 and 3.5 times in 20 mW/cm2 group and 30 mW/cm2 group respectively compared with control group, there were significant differences among them (P < 0.05). NBT method shows that the activity of SOD were 68.2%, 46.2% and 36.0% of 10, 20 and 30 mW/cm2 groups respectively compared with control group, there were significant differences among them too (P < 0.01).
CONCLUSION:Microwave Radiation could up-regulate the levels of MAD) while down-regulate the SOD activity.
