Chemical Modification of RBC Surface Antigen with Methoxy Polyethylene Glycol.
- Author:
Jun Soo BAE
1
;
Mi Won HWANG
;
Il Tae KIM
;
Chae Seung LIM
;
Kyung Ran MA
;
Young Kee KIM
;
Kap No LEE
;
Do Hyung KIM
;
Si Myung BYUN
Author Information
1. Department of Clinical Pathology, College of Medicine, Korea University, Seoul, Korea
- Publication Type:Original Article
- Keywords:
Methoxy polyethylene glycol;
ABO;
Rh(D) antigen;
agglutinability;
artificial blood
- MeSH:
Antigens, Surface*;
Blood Group Antigens;
Blood Substitutes;
Healthy Volunteers;
Immune Sera;
Polyethylene Glycols*;
Polyethylene*
- From:Korean Journal of Clinical Pathology
1999;19(6):723-728
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Today, blood group antigens are a strong barrier of safe transfusion. We evaluated the change of agglutinability of antibody to RBC surface antigen before and after activated methoxy polyethylene glycol (mPEG) modification. METHODS: We collected blood from healthy volunteers and the blood were treated by activated mPEG (MW 5,000, Sigma, USA). Agglutinability of RBC was measured using anti-sera (Green Cross, Korea) in ABO and Rh(D) groups, and compared the agglutinability changes before and after mPEG treatment. RESULTS: The agglutinability of Rh(D) surface antigen (n=20) was disappeared after mPEG treatment. However, ABO antigens showed variable agglutinability against antisera, some of which showed no change at all. CONCLUSIONS: In the case of Rh(D) antigen, it would be useful to apply mPEG treated RBCs for clinical use, if the safety problem were solved. But in the case of ABO antigen, the more evaluation of the condition of reaction and the concentration of mPEG should be needed.