Effects of vitamin C combined with arsenic trioxide on the apoptosis of Hep-2 cell.
- Author:
Weimin XU
1
;
Chenghua SHU
;
Jin HU
;
Yuhua YE
Author Information
1. Department of Otolaryngology, Renmin Hospital of Tongshan County, 437600, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenic Trioxide;
Arsenicals;
pharmacology;
Ascorbic Acid;
pharmacology;
Cell Line, Tumor;
Drug Synergism;
Humans;
Oxides;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(4):171-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study synergistic effect of Vitamin C (VitC) combined with arsenic trioxide (As2 O3)-on human laryngocarcinoma Hep-2 cell line apoptosis.
METHOD:Human laryngocarcinoma cell line Hep-2 was cultured in vitro, and incubated with As2 O3 or jointly with VitC. The inhibition ability of Hep-2 cells was determined by methyl thiazolyl tetrazolium (MTT) assay, while the apoptosis was mensurated by the flow cytometry with Annexin-V/PI.
RESULT:Compared with the use of As2 O3 separately, the combination of As2 O3 and VitC could increase the inhibition ability and the apoptosis rate of Hep-2 cells markedly (P < 0.05).
CONCLUSION:Combination of As2 O3 with VitC could markedly increase arsenic trioxide (As2 O3)-induced apoptosis of Hep-2 cells.
