HIF-1alpha and CA IX expression in nasal polyps and study on their correlation.
- Author:
Hai LIN
1
;
Dong LIN
;
Xianming CHEN
;
Xian ZHANG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Fuzhou General Hospital of Nanjing Command of PLA, Fuzhou, 350025, China. linh96@163.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antigens, Neoplasm;
metabolism;
Carbonic Anhydrase IX;
Carbonic Anhydrases;
metabolism;
Case-Control Studies;
Female;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Male;
Middle Aged;
Nasal Polyps;
metabolism;
pathology;
Young Adult
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(17):776-778
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the expression and relationship between hypoxia inducible factor-1alpha (HIF-1alpha) and carbonic anhydrase IX (CA IX) in nasal polyps, and to explore the role of HIF-1alpha and CA IX in the pathogenesis of nasal polyps.
METHOD:Twenty-eight tissues of nasal polyps and 12 tissues of normal inferior turbinate mucosa were collected in this study. And Immunohistochemistry method was used to detect the expression of HIF-1alpha and CA IX in the two groups.
RESULT:The numbers and intensity of positive stained cells of HIF-1alpha and CA IX were higher in nasal polyps. IOD of HIF-1alphaa and CA IX (10(3)/HP) in the nasal polyps were respectively 21.76 +/- 3.52, 26.87 +/- 4.60 compared with 3.37 +/- 1.65, 3.25 +/- l.20 in the control group by image analysis. There were significant differences between the two groups (P < 0.05). Spearman correlation test showed that there was a close correlation between the HIF-1alpha and CA IX expression in nasal polyps (r = 0.820, P < 0.01).
CONCLUSION:The results showed that HIF-1alpha and CA IX were over expressed in nasal polyps, and there was a close correlation between them. Hypoxia was found in nasal polyps and it could become more severe under infection, inflammation and energy metabolic disorder which consumed a great deal of oxygen. HIF-1alpha was over expressed in nasal polyps, which activate and promote the expression for CA IX, in order to keep the normal pH of cells for adapting the hypoxic microenvironment and sustain persistent growth and hyperblastosis of cells in the hypoxic areas. At last the internal circle was forming, which accelerate the formation of nasal polyps.
