The role of intracellular Ca2+ release in olfactory signal transduction.
- Author:
Mu XIAN
1
;
Demin HAN
;
Luo ZHANG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
metabolism;
Calcium Signaling;
Cells, Cultured;
Cyclic AMP;
metabolism;
Mice;
Mice, Inbred BALB C;
Olfactory Receptor Neurons;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(20):940-944
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To setup the real time monitor system of the concentration of free intracellular calcium ([Ca2+]i) of olfactory receptor neurons (ORNs) cultured from olfactory epithelium explant, and to analyze the role of several important components in olfactory signal transduction.
METHOD:The [Ca2+]i of the cultured ORNs was determined by fluorescence microscopy using the fluorescent calcium indicator, Fura-2 AM, and calculated by means of dual-wavelength ratiometric method. Forskolin and IBMX were used to stimulate the cultured ORNs respectively. The source of corresponding [Ca2+]i elevation was studied by the depletion of extracellular or intracellular calcium.
RESULT:The [Ca2+]i of silent ORNs was (58.5 +/- 12.8) nmol/L. Forskolin or IBMX stimulation led to reversible accumulation of [Ca2+]i in the ORNs. The [Ca2+]i change was abolished with the removal of extracellular Ca2+ and un-affected by treatment with thapsigargin.
CONCLUSION:A system to visualize and quantify [Ca2+]i of the ORNs was established. [Ca2+]i of the ORNs was regulated by second messenger gated calcium channels.
