Relationship between hypermethylation of Runx3 gene and the development and metastasis of laryngeal carcinoma.
- Author:
Jing TANG
1
;
Weiliang BAI
;
Wenyue JI
;
Hong GAO
;
Jiao LIU
Author Information
1. Department of Otorhinolaryngology, the Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
genetics;
metabolism;
pathology;
Core Binding Factor Alpha 3 Subunit;
genetics;
metabolism;
DNA Methylation;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Laryngeal Neoplasms;
genetics;
metabolism;
pathology;
Lymphatic Metastasis;
Male;
Middle Aged;
RNA, Messenger;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(18):834-837
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationships between hypermethylation of human runt-related transcription factor 3 (Runx3) gene promoter and laryngeal squamous cell cancer.
METHOD:Promoter hypermethylation and mRNA expression were detected by methylation-specific PCR and RT-PCR.
RESULT:The expression of Runx3 gene mRNA detected in laryngeal carcinoma (1.62 +/- 1.01) was lower than that in adjacent tissues samples (5.66 +/- 2.07) (t = 10.72, P < 0.01). No methylation of Runx3 promoter was found in adjacent tissues samples. But hypermethylation was found in 95.0% (38/40) of the laryngeal carcinoma specimens. The rate of methylation of Runx3 promoter in laryngeal carcinoma was higher than that in adjacent tissues (P < 0.01). The Runx3 mRNA were down-regulated in lymphnode metastasis or poorly differentiated groups, but the Runx3 promoter methylation were detected in those groups markedly.
CONCLUSION:Hypermethylation of Runx3 promoter is one of the inactivation re-seasons in laryngeal squamous cell carcinoma, and the decreasing of Runx3 mRNA expression may be related to lymph node metastasis and development of laryngeal squamous cell carcinoma.
