The expression of DNMT1 in laryngeal squamous cell carcinoma and its clinical significance.
- Author:
Jin WANG
1
;
Jun LI
;
Xin SUN
;
Mingming ZHANG
Author Information
1. Department of Otolaryngology, Shengjing Hospital, China Medical University, Shenyang, 110004, China. wangjin068427@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
metabolism;
pathology;
DNA (Cytosine-5-)-Methyltransferase 1;
DNA (Cytosine-5-)-Methyltransferases;
genetics;
metabolism;
Female;
Humans;
Laryngeal Neoplasms;
metabolism;
pathology;
Male;
Middle Aged;
Neoplasm Staging;
Smoking
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(24):1105-1111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of DNMT1 in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinicopathological factors.
METHOD:Ninety-six cases of LSCC samples were detected by immunohistochemical staining to analyze the expression of DNMT1 protein, while 66 cases of them were detected by real-time PCR. And the relationship between the expression of DNMT1 mRNA and clinicopathologic factors was then analyzed.
RESULT:The expression of DNMT1 mRNA in LSCC were up-regulated (P < 0.05). The positive rate of DNMT1 protein expression in LSCC and in peri-cancer tissue were 100% (96/96) and 36% (8/22) respectively, which showed a significant difference (P < 0.05). DNMT1 mRNA expression wasn't correlated with patients' age, gender, T stage and lympha node metastasis, but was associated with smoking habit (P < 0.05, respectively).
CONCLUSION:DNMT1 may initiate the oncogenesis of LSCC by increasing expression, and smoking habit may induce the expression of DNMT1 gene.
