Experiment studies on growth and proliferation of Hep-2 cells by silence of TRF2 gene.
- Author:
Luyan JIAO
1
;
Yong XU
;
Zezhang TAO
Author Information
1. Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
Cell Line, Tumor;
Cell Proliferation;
Humans;
RNA Interference;
RNA, Small Interfering;
genetics;
Telomeric Repeat Binding Protein 2;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(24):1131-1135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of RNA interference silencing telomere repeat factor 2 by observing Hep-2 cells' proliferation and apoptosis in larynx carcinoma cell line.
METHOD:A recombinant plasmid containing a single shRNA (shTRF2) was constructed. The expression of TRF2 gene was detected by PCR and cell proliferation was examined using CCK-8. Hep-2 cells' apoptosis was detected by flow cytometer (FCM).
RESULT:After treatment with shTRF2, the expression of TRF2 was distinctively depressed, and Hep-2 cells proliferation was obviously inhibited. Compared with control and negative group, cells with treatment of RNAi exhibited significantly more apoptosis.
CONCLUSION:Using RNA interference technique to silence TRF2 gene is effective on inhibiting cancer cells' proliferation and help to induce cancer cells' apoptosis.
