Impact of RelA antisense oligonucleotides on laryngeal carcinoma Hep-2 cell proliferation.
- Author:
Song PAN
1
;
Jingzhi WAN
;
Lilian WU
;
Ji ZHAO
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, the Affiliated Hospital of Xianning University, Xianning, 437100, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Laryngeal Neoplasms;
genetics;
pathology;
Oligonucleotides, Antisense;
genetics;
pharmacology;
Transcription Factor RelA;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(24):1135-1137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the impacts of RelA antisense oligonucleotides on proliferation in laryngeal carcinoma Hep-2 cell.
METHOD:RelA antisense oligonucleotides was designed, which was transferred into laryngeal carcinoma Hep-2 cell. MTT was used to detect the growth-inhibiting ratio at different transferred timepoints. Hep-2 cell which was transferred 48 h was used to do colony assay, and expression of RelA was detected by Reverse Transcription PCR and Western blot.
RESULT:MTT results showed that RelA antisense oligonucleotides could significantly suppress the proliferation of Hep-2 cell, and the suppression-ratio elevated with time. There were statistical difference compared with control groups. The number of cells colony was reduced in RelA antisense oligonucleotides group compared with control groups, which had statistic significance. RT-PCR and Western blot results demonstrated that RelA antisense oligonucleotides could significantly inhibit the expression of messenger RNA and protein in Hep-2 cell.
CONCLUSION:RelA antisense oligonucleotides can inhibit the expression of messenger RNA and protein, and induce the cell proliferation and increase the number of cells colony in Hep-2 cell.
