Effects of EGCG on the nasopharyngeal carcinoma cell line CNE-2 and the expression of related gene.
- Author:
Xun LEI
1
;
Zhongyu KONG
;
Caiwen FAN
;
Shijiang YI
;
Qiu XIANG
;
Qianghe LIU
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin, 541001, China. kongzhongyuilql@163.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma;
Caspase 3;
metabolism;
Catechin;
analogs & derivatives;
pharmacology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(15):697-700
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of epigallocatechin-3-gallate (EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2, Bax and Caspase-3 in the cell line which treated with EGCG.
METHOD:MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle. Hoechst33258 fluorescence staining was adopted to study cell apoptosis. RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3.
RESULT:EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosis with dose-independent relationship. EGCG could suppress the expression of Bcl-2 and induce expression of Bax, Caspase-3.
CONCLUSION:EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells, which may be through regulating the expression of cell proliferation and apoptosis genes involved.
