Detection and correlation of lipopolysaccharide, vitamin D receptor and matrix metalloproteinase-9 in the middle ear cholesteatoma.
- Author:
Yongling LI
1
;
Zhiwen XU
;
Longcheng ZHANG
;
Chaokun QUAN
;
Xinran LIN
Author Information
1. Department of Otolaryngology Head and Neck Surgery,No 303 Hospital of PLA, Nanning, 530021, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Cholesteatoma, Middle Ear;
metabolism;
pathology;
Female;
Humans;
Lipopolysaccharides;
metabolism;
Male;
Matrix Metalloproteinase 9;
metabolism;
Receptors, Calcitriol;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(16):739-746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and concentration of lipopolysaccharide (LPS) and matrix metalloproteinase-9 (MMP-9) in middle ear cholesteatoma and discuss their relations.
METHOD:Twenty-nine cases of middle ear cholesteatoma tissue, 18 cases of external auditory canal tissue were detected by limulus amebocyte lysate assay (LAL-assay), and expression of MMP-9 protein in formalin-fixed, paraffin-embedded tissues was detected by immunohistochemical method.
RESULT:The concentrations of LPS in cholesteatoma were higher than that in external auditory canal tissues. In group of cholesteatoma: M = 0.739 0, IQR = 0.6203, and in group of external auditory canal tissues: M = -0.2538, IQR = 1.1692 (P < 0.01). In cholesteatoma groups, in extensive type: M = 0.8403, IQR = 0.5254; in localized type: M = 0.4048, IQR = 0.6139, the concentrations of LPS were higher in extensive cholesteatoma in comparison with localized cholesteatoma (P < 00.05). In cholesteatoma epithelium samples, MMP-9 were 79.3%. Compared with external auditory canal epithelium, the expression of MMP-9 was higher in middle ear cholesteatoma epithelium (P < 0.05). There was no significant difference in the expression of MMP-9 between two types of cholesteatoma epithelium (P > 0.05). LPS, MMP-9 weren't significantly correlated by Spearman test.
CONCLUSION:LPS was responsible for middle ear cholesteatoma and its related bone erosion. MMP-9 was related to the development of middle ear cholesteatoma. There's no correlation between LPS and MMP-9.
