The influence of autophagy-related genes about X-Ray on nasopharyngeal carcinoma CNE2 and CNE2/DDP cells.
- Author:
Feng LI
;
Dewei CUI
;
Weihua XU
;
Minglang HUI
;
Leifeng LIU
;
Haitao QIU
;
Wenjie XIAO
- Publication Type:Journal Article
- MeSH:
Apoptosis Regulatory Proteins;
genetics;
Autophagy;
Beclin-1;
Carcinoma;
Cell Cycle;
Cell Line, Tumor;
radiation effects;
Dose-Response Relationship, Radiation;
Humans;
Membrane Proteins;
genetics;
Microtubule-Associated Proteins;
genetics;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
genetics;
Radiation Tolerance;
X-Rays
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(6):547-551
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity.
METHOD:Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3β (MAPLC3β) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining.
RESULT:CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3β in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3β in CNE2/DDP was lower than that in CNE2 cells (P<0. 05).
CONCLUSION:Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.
