Tumor suppressive effect and relative mechanisms of tea polyphenol on nasopharyngeal carcinoma cells.
- Author:
Mengqiu TIAN
;
Dongjie YUAN
;
Shixing ZHENG
;
Qingyu LI
;
Shujing SHI
;
Zhiwen XU
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma;
Cell Line, Tumor;
drug effects;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
pathology;
Polyphenols;
pharmacology;
Tea;
chemistry
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(6):552-556
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and mechanism of tea polyphenol (TP) on the proliferation, apoptosis, migration and invasion of nasopharyngeal carcinoma(NPC) cell line HONEl.
METHOD:After treated with different concentration of tea polyphenol, CCK-8 assay, fluorescent staining, cell scratching assay and transwell assay were applied to detect the effect of tea polyphenol on the HONE1 cells. Furthermore, the expression of protein VEGF was investigated by flow cytometry assay.
RESULT:It was found that tea polyphenol could inhibit NPC cell proliferation significantly in a dose-dependent manner, however, little impact was observed in normal nasopharyngeal epithelial cell line NP69. Furthermore, it was demonstrated by fluorescent staining assay that tea polyphenol could induce NPC cell apoptosis, and cell scratching assay and transwell assay showed that tea polyphenol could inhibit cell migration and invasion.
CONCLUSION:Tea polyphenol can significantly inhibit cell proliferation, induce cell apoptosis and decreased the migration and invasion ability of NPC cells in vitro. Tea polyphenol might be a tumor suppressor of NPC cells.
