Effect of microRNA-205 on proliferation of laryngeal carcinoma cell line Hep-2.
- Author:
Xiaotian LI
;
Wei LI
;
Xuejun JIANG
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
Humans;
Laryngeal Neoplasms;
metabolism;
pathology;
MicroRNAs;
antagonists & inhibitors;
metabolism;
PTEN Phosphohydrolase;
metabolism;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(9):831-834
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of microRNA-205 (miRNA-205) on proliferation of laryngeal carcinoma cell line Hep-2.
METHOD:The expressions of miRNA-205 in 27 cases laryngeal carcinoma tissues and adjacent normal tissues were detected by Real-time quantitative PCR, the expression of PTEN protein was detected by Western blot. The expressions of PTEN were detected by Western blot after miRNA-205 inhibitor or miRNA-205 mimics was transfected into Hep-2 cells and Hep-2 cells proliferation was measured by CCK-8 kit.
RESULT:The expression level of miRNA-205 was significantly higher in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01), and the expression of PTEN protein was lower in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01). The proliferation rate of Hep-2 cells was decreased significantly and the expression of PTEN protein in Hep-2 cells was increased significantly after miRNA-205 inhibitor was transfected into (P < 0.01), and the proliferation rate of Hep-2 cells was increased significantly and the expression of PTEN protein in Hep-2 cells was decreased significantly after miRNA-205 mimics was transfected into (P < 0.01).
CONCLUSION:miRNA-205 might promote the proliferation of Hep-2 cells by regulating the expression of PTEN.
