An in vitro study on substance P-stimulated neuro-immune mechanism of mast cell degranulation.
- Author:
Qiuting LI
;
Changqing ZHAO
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
Cell Degranulation;
Cells, Cultured;
Culture Media;
chemistry;
Histamine;
metabolism;
Interleukin-3;
pharmacology;
Interleukin-4;
pharmacology;
Mast Cells;
cytology;
metabolism;
Mice;
Stem Cell Factor;
pharmacology;
Substance P;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(12):1118-1120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The goal of this study was to study the mechanism of substance P (SP)-mediated the neural control of mast cell (MC) degranulation.
METHOD:Bone marrow mast cells from mice were cultured with stem cell factor (SCF), IL-3 and IL-4 (group A) and SCF, IL-3 (group B) for four weeks. Then the cells were harvested and reserved for studies. Western Blot hybridization technique was used to detect the expression of FcεR I α and NK-1R on MCs from the two groups. Then such cells were activated with SP (0, 0. 01, 0. 10, 1. 00, 10. 00 µg/ml, respectively) for 30 min. The histamine released into the supernatant and stored in the protoplasm was quantified by enzyme linked immunosorbent assay (ELISA). And the percentage of histamine release was calculated as a percent of total histamine content.
RESULT:The expressions of FcεR I α and NK-1R on these mast cells in group A were statistically higher than in group B (P<0. 05). The MCs from two groups can be actived when stimulated by SP, but the level of MC degranulation in group A was higher than group B (P<0. 05).
CONCLUSION:Neuropeptide may stimulate MC degranulation through immunological and non-immunological pathways. In summary, the current study provides us with better understanding of the mechanism of neuropeptide-controlled MC deranulation, and this should be helpful for the further research involved in the mechanism and treatmemt of airway hyper-reactivity.
