The expression of PKM2 and HIF-1α in laryngeal squamous cell carcinoma and its clinical indication.
- Author:
Xiaoxiao LIU
;
Chunguang SHAN
;
Ou XU
;
Jingmiao WANG
;
Haizhong ZHANG
- Publication Type:Journal Article
- MeSH:
Biomarkers, Tumor;
metabolism;
Carcinoma, Squamous Cell;
metabolism;
Carrier Proteins;
metabolism;
Head and Neck Neoplasms;
metabolism;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Laryngeal Neoplasms;
metabolism;
Lymphatic Metastasis;
Membrane Proteins;
metabolism;
Squamous Cell Carcinoma of Head and Neck;
Thyroid Hormones;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(13):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The purpose of this study was to investigate the expression of PKM2 and HIF-1α in laryngeal squamous cell carcinoma and to analyze their correlation in laryngeal squamous cell carcinoma.
METHOD:Total 37 laryngeal carcinoma samples and para-carcinoma tissues were taken from the patients who accepted operation treatment in the Second Hospital of HeBei Medical University from 06/2013 to 06/2014. The protein expression levels of PKM2 and HIF-1α were detected with SP immunohistochemical methods. The data were analyzed by the SPSS 13.0 statistical software.
RESULT:The positive expression of PKM2 in laryngeal carcinoma tissues and adjacent tissues were 62.16% and 13.15%. The difference was statistically significant (P < 0.01). The positive expression of HIF-1α in laryngeal carcinoma tissues and adjacent tissues were 64.86% and 21.62%. The difference was statistically significant (P < 0.01). The positive expression of PKM2 and HIF-1α in well differentiated laryngeal squamous cell carcinoma were both 47.83%, while in medium and poorly differentiated laryngeal squamous cell carcinoma were 85.71% and 92.86% respectively. The difference was statistically significant (P < 0.05). The positive expression of PKM2 and HIF-1α in patients with lymph metastasis were 90.00% and 100.00% respectively, 51.85% in those without lymph metastasis, the difference was statistically significant (P < 0.05). The rate of HIF-1α positive expression in I-II stage was 53.85%, 90.91% in III-IV stage. The difference between the two groups was statistically significant (P < 0.05). The expression of PKM2 and HIF-1α had no relationship with the age and smoking (P > 0.05). The expression of PKM2 was positively related with HIF-1α in laryngeal squamous cell carcinoma (P < 0.01).
CONCLUSION:The expression of PKM2 and HIF-1α are related with the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma. It provides a certain theoretical basis for laryngeal cancer diagnosis and screening to measure the expression of PKM2 and HIF-1α as biological indicators.
