Expression and methylation status of IGFBP-rP1 gene in laryngocarcinoma.
- Author:
Junlan HU
;
Ruili ZHAO
;
Ganxun WU
;
Jingtian WANG
- Publication Type:Journal Article
- MeSH:
Adenocarcinoma;
genetics;
metabolism;
pathology;
Aged;
DNA Methylation;
Female;
Humans;
Insulin-Like Growth Factor Binding Proteins;
genetics;
metabolism;
Laryngeal Neoplasms;
genetics;
metabolism;
pathology;
Male;
Middle Aged
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(24):1352-1359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the promoter methylation and protein expression of insulin-like growth factor binding protein-related protein 1(IGFBP-rP1) in laryngeal squamous cell carcinoma (LSCC).
METHOD:Methylation specific PCR (MSP) approach and immunohistochemistry methods were used to examine the methylation status and protein expression of IGFBP-rP1 in 45 samples of laryngeal carcinoma and 18 samples of tissues beside cancer.
RESULT:For the promoter site, methylation frequency of IGFBP-rP1 in tumor specimens (33.3%, 15/45) was significantly higher than that in corresponding normal tissues (5.6%, 1/18) (P < 0.05). The protein expression of IGFBP-rP1 in tumor tissues was significantly higher than that in corresponding normal tissues (P < 0.05) and was inversely correlated with its methylation status of promoter.
CONCLUSION:Epigenetic silencing of IGFBP-rP1 gene expression by promoter hypermethylation may play an important role in LSCC.
