Inhibitory effect of arsenic trioxide combined with cisplatin on human nasopharyngeal carcinoma xenograft and DAPK in nude mice.
- Author:
Xueqin HUANG
1
;
Xiaogang WANG
;
Junli HU
;
Hui ZHOU
;
Keyuan ZHOU
;
Yuefei ZHANG
Author Information
1. Guangdong Medical College, Zhanjiang, 524023, China. hxqcong@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Arsenic Trioxide;
Arsenicals;
administration & dosage;
pharmacology;
Carcinoma;
Cell Line, Tumor;
Cisplatin;
administration & dosage;
pharmacology;
Death-Associated Protein Kinases;
metabolism;
Gene Expression Regulation, Neoplastic;
Humans;
Mice;
Mice, Nude;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Oxides;
administration & dosage;
pharmacology;
Xenograft Model Antitumor Assays
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(9):479-483
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effect of Arsenic Trioxide (As2O3) combined with diamminedichloroplatinum (DDP) on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and to explore the possible effect mechanisms of the antitumor.
METHOD:The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group, As2O3 group, DDP group and As2O3 + DDP group. The effect of antitumor on each group was studied. The specimen obtained from the mice were detected by optical microscope and tdt-mediated dutp rock end labeling (tunel) method. Expression of DAPK was detected by real time-PCR and immunohistochemistry.
RESULT:As2O3 group and AS2O3 + DDP group could obviously inhibit the growth of tumor, induce the apoptosis of human naso pharyngeal carcinoma cell and up-regulate the expression of RASSF1A.
CONCLUSION:As2O3 can greatly inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, which were related to the induced apoptosis of human nasopharyngeal carcinoma cell and up-regulated expression of DAPK Combination of As2O3 with DDP seem to be more effective.
