Apoptosis of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism.
- Author:
Zhenhe HUANG
1
;
Yuefei ZHANG
;
Donghui JIANG
;
Xueqin HUANG
;
Bo HUANG
;
Guoqing LUO
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Affliated Hospital of Guangdong Medical College, Zhanjiang 524023, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Artemisinins;
pharmacology;
Carcinoma;
Caspase 3;
metabolism;
Cell Line, Tumor;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(13):717-720
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Dihydroartemisinin is a traditional anti-malarial drug, a derivative of the artemisinin, it has anti-tumor activity of a variety of tumor cells. This study investigated the effect of growth inhibition of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism.
METHOD:The effect of DHA on the cell proliferation of CNE-2 was detected by CCK-8 assay with different concentrations and time. The effects of DHA on the cell apoptosis of CNE-2 were detected by Annexin V-FITC assay through flow cytometry and caspase-3 activity assay.
RESULT:CCK-8 experimental results show that CNE-2 cell proliferation was suppressed with DHA treatment, as compared with the control group. DHA could induce marked apoptosis in CNE-2 by apoptosis assay, as compared with the control group, The percentages of apoptotic cells increased along with the increase of DHA concentrations in CNE-2; The activity of caspase-3 was increased following DHA treatment in a dose-dependent manner.
CONCLUSION:DHA could effectively inhibit proliferation and induce apoptosis of human nasopharyngeal carcinoma cells line CNE-2, the possible mechanism DHA induce apoptosis of CNE-2 cells by upregulating the expression of caspase-3.
