The inhibiting role of recombinant plasmid PGCsi-AQP1 on laryngeal carcinoma in vivo.
- Author:
Guimei GUAN
;
Dongdong ZHU
;
Zhen DONG
;
Jichao SHA
- Publication Type:Journal Article
- MeSH:
Animals;
Aquaporin 1;
therapeutic use;
Cell Line, Tumor;
Laryngeal Neoplasms;
therapy;
Liposomes;
Male;
Mice;
Mice, Inbred BALB C;
Neoplasm Transplantation;
Plasmids;
RNA Interference;
RNA, Small Interfering;
Random Allocation;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(21):1886-1893
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct a kind of recombinant plasmid PGCsi-AQP1 delivery with DOPC and explore the inhibit effect of laryngeal carcinoma by RNAi targeting AQP1 in vivo.
METHOD:Male BALB/c mice, 6 weeks of age transplanted with laryngeal carcinoma cell line Hep-2, four groups were divided randomly: Tail vein injection group (TVIG), Carcinoma around injection group (CAIG), negative control group (NCG) and blank control group (BCG). The recombinant plasmid PGCsi-AQP1 delivery with DOPC were inject into tail vein or surrounding tumor. HE pathological slides and tumor size were observed and inhibitory rate was figured up. The level of AQP1 protein expression and high microvessel density were detected by Immunohistochemical staining (IHC).
RESULT:We constructed BALB/c mice models of laryngeal carcinoma successfully (1) HE staining: cell putrescence, nuclear pyknosis and apoptotic bodies were more in the tumor tissues of experimental groups than two control groups. (2) The total volumes of tumor in experimental group were both smaller than in two control groups (P < 0.01). The inhibition rate of TVIG and CAIG were 52.4% and 53.5% respectively and there was no significant difference (P > 0.05). (3) IHC: the AQP1 positive cells and microvessel density in TVIG and CAIG were both less than in two control groups (P < 0.01).
CONCLUSION:Neutral lipsomes DOPC could help carriaging the recombinant plasmid PGCsi-AQP1 to tumor and then play an inhibit role in laryngeal carcinoma tissue by RNAi targeting AQP1 in vivo.
