The effect of oxidative stress on aorta endothelial cell in chronic intermittent hypoxia animal model.
- Author:
Jie LI
1
;
Bing LI
;
Xiang YANG
;
Hailan MO
Author Information
1. Department of Otorhinolaryngology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Aorta;
metabolism;
Disease Models, Animal;
Endothelial Cells;
metabolism;
Hypoxia;
blood;
Malondialdehyde;
blood;
Nitric Oxide;
blood;
Nitric Oxide Synthase Type III;
blood;
Oxidative Stress;
physiology;
Random Allocation;
Rats;
Superoxide Dismutase;
blood
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(9):411-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To make a chronic intermittent hypoxia (CIH) animal model and explore the relationships of oxidative stress and the injury of aorta endothelial cell on the CIH rat model.
METHOD:Twenty-four sex maturation rat were randomly Divided into unhandled control group(normal feeding for 4 weeks, UC group), CIH group (CIH for 4 weeks), reoxygenation group (normal feeding for 4 weeks after CIH for 4 weeks, RH group). Determined the activity of superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) in blood serum of all groups. Dislodged rat's aorta, observed the appearance of aorta endothelial cell and the expression of endothelial nitric oxide synthase (eNOS).
RESULT:Compared with UC group, in CIH group the activities of SOD and NO were significantly lower, but MDA significantly higher (all of P < 0.05 respectively), the appearance of aorta endothelial cell changed, the expressions of eNOS lower. There were not significantly change in UC group and RH group (P > 0.05).
CONCLUSION:Oxidative stress played a role in the injury of aorta endothelial cell on ob chronic intermittent hypoxia rat modle.
