Effects of 5-Aza-2'-deoxycitydine and trichostatin A on expression and methylation of CHFR in human laryngreal carcinoma cell line.
- Author:
Lixia HE
1
;
Wenyue JI
;
Jing YANG
;
Xudong ZHAO
Author Information
1. Department of Otorhinolaryngology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
- Publication Type:Journal Article
- MeSH:
Azacitidine;
analogs & derivatives;
pharmacology;
Cell Cycle Proteins;
genetics;
metabolism;
DNA Methylation;
drug effects;
Decitabine;
Gene Expression;
drug effects;
Hep G2 Cells;
Humans;
Hydroxamic Acids;
pharmacology;
Laryngeal Neoplasms;
metabolism;
Methylation;
drug effects;
Neoplasm Proteins;
genetics;
metabolism;
Poly-ADP-Ribose Binding Proteins;
Promoter Regions, Genetic;
Ubiquitin-Protein Ligases
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(9):418-421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of 5-Aza-2'-deoxycitydine(5-Aza-dC) and trichostatin A (TSA) on the expression and methylation of CHFR in human laryngeal carcinoma cell line.
METHOD:The mRNA expression and promoter hypermethylation and were detected by Realtime fluro-genetic quantitative PCR and methylation specific PCR in Hep-2 cell line, which were cultured in vitro and then treated with different concentrations of 5-Aza-dC and TSA.
RESULT:Compared with the control team, 5-Aza-dC alone reactivated expression of the CHFR in Hep-2 cell line (1.75 +/- 0.21). TSA had no effect on gene expression (1.05 +/- 0.13). The combined treatment with 5-Aza-dC and TSA increased gene expression (2.15 +/- 0.18). The cell lines showed a characteristic DNA methylation status. 5-Aza-dC and combined 5-Aza-dC and TSA resulted in demethylation of CHFR. In contrast, TSA alone did not affect the DNA methylation status of CHFR.
CONCLUSION:Hypermethylation of CHFR gene promoter is a common event in the occurrence and development of laryngeal carcinoma. The promoter aberrant methylation of CHFR is a main cause for down-expression of CHFR. After either treatment with 5-Aza-dC alone or in combination with TSA, the expression of CHFR is up-regulated duo to the reversal methylation. It can be a new idea to the therapy of laryngeal carcinoma.
