Effects of Bmi-1 RNAi gene on laryngeal carcinoma Hep-2 cells.
- Author:
Xiaobao YAO
1
;
Xiaoxia WANG
;
Shaoqiang ZHANG
;
Hongliang ZHU
Author Information
1. Department of Otolaryngology, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China. yaoxiaobao@sohu.com
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
genetics;
Genetic Vectors;
Humans;
Laryngeal Neoplasms;
genetics;
metabolism;
pathology;
Neoplasm Invasiveness;
genetics;
Polycomb Repressive Complex 1;
metabolism;
RNA, Small Interfering;
metabolism;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(12):550-557
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct the Bmi-1 RNAi expression vector and investigate its influence on the proliferation and invasiveness of laryngeal carcinoma Hep-2 cells.
METHOD:The recombinant vshRNA-Bmi-1 plasmid of Bmi-1 RNAi was constructed by the lentiviral expression system, pHelper1.0/pHelper2.0/pGCL2GFP. Bmi-1mRNA and protein expressions of stably transfected laryngeal carcinoma cells were identified tespectively by real-time PCR and Western blot analyses. The changes of the proliferation and invasiveness of laryngeal carcinoma were detected by clone formation test and an invasion assay.
RESULT:The Bmi-1 mRNA expressions of stably transfected laryngeal carcinoma Hep-2 cells were significantly decreased. The expression of Bmi-1 protein in laryngeal carcinoma Hep-2 cells was significantly decreased. And the inhibitory rates were 79% and 88% respectively. Whereas the proliferation and invasiveness of Hep-2 cells were significantly reduced.
CONCLUSION:The Bmi-1 RNAi expression vector were constructed successful. Reduced Bmi-1 expression of Hep-2 cells demonstrated the role of Bmi-1 RNAi in restraining proliferation and invasiveness of laryngeal carcinoma cells.
