Study and analysis on the quantitive detection of EBV-DNA in adenoidal hypertrophic and tonsillitis tissues of children.
- Author:
Xiaotong ZHANG
1
;
Hongyue LI
;
Xuemei LIU
;
Qing ZHANG
;
Hui LIU
;
Xiangling WANG
;
Ying LING
Author Information
1. Department of Otolaryngology, the Second Teaching Hospital, Medical College of Xi'an Jiaotong University, Xi'an, 710004, China. xiaotong3535@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adenoids;
pathology;
virology;
Adolescent;
Child;
Child, Preschool;
DNA, Viral;
isolation & purification;
Epstein-Barr Virus Infections;
pathology;
Female;
Herpesvirus 4, Human;
Humans;
Hypertrophy;
pathology;
virology;
Male;
Palatine Tonsil;
pathology;
virology;
Tonsillitis;
pathology;
virology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(24):1108-1111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the epidemiology of EBV in adenoidal hypertrophy and chronic tonsillitis and discuss the affection of EBV on the nosogenesis of adenoidal hypertrophy and tonsillitis of children.
METHOD:Fifty-two children with chronic tonsillitis and/or adenoidal hypertrophy had the operations of the tonsillectomy and/or the adenoidectomy. These tissues resected and plasma of all cases were detected to find EBV-DNA by RQ PCR.
RESULT:The infection rate of EBV in the tissues of adenoidal hypertrophy and tonsillitis of children was 51.9%. The boys' infection rate of EBV was 50.0%, and the girls' infection rate of EBV was 55.6%, which had not significantly different. The EBV infection rate in the tissues of tonsillitis was 40.4%, The EBV infection rate in the tissues of adenoidal hypertrophy was 48.9%, which had not significant difference. The school age group (7- to 14-years-old) presented higher infection rate of EBV in the tissues of adenoid and tonsil (65.5%) than the pre-school children group (2- to 6-years-old) (34.8%). Comparing the copies numbers of EBV-DNA in the different degrees of adenoidal hypertrophy, we found that the copies numbers of EBV-DNA in the severe hypertrophy group were higher than the midrange and slight hypertrophy groups (P<0.05). Meanwhile we detected EBV-DNA in these childrens' blood plasma by RQ-PCR. No blood plasma was detected EBV-DNA copies higher than normal (< 1 x 10(3) copies/ml).
CONCLUSION:The tissues of adenoidal hypertrophy and tonsillitis had same sensitivity to EBV. There was not significant difference between the infection rates of the boys and girls with adenoidal hypertrophy and/or tonsillitis. With these children growing up and the course of diseases prolonging, the infection rate of EBV increased correspondingly. There was a certain correlation between the hypertrophy of adenoid and EBV. There were no EBV-DNA fragments in blood plasma of the children with adenoidal hypertrophy and/or tonsillitis. So there were essential different between benign hyperplasia and nasopharyngeal carcinoma.
