Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors.
- Author:
Haili LÜ
1
;
Qiuhang ZHANG
;
Bo YAN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing, 100053, China. lvhaili@hotmail.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
Cell Line, Tumor;
Gene Expression Regulation, Neoplastic;
Humans;
Oligodeoxyribonucleotides, Antisense;
genetics;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Proto-Oncogene Proteins c-myc;
metabolism;
STAT3 Transcription Factor;
genetics;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(4):155-157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide (ASODN).
METHOD:The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Bcl-2, Bax and C-Myc were detected by Western blot and PCR.
RESULT:Western blot and PCR results demonstrated that Stat3 ASODN could significantly increased the expression of Bax and decreased the expression of Bcl-2 and C-Myc when the concentration of antisense oligodeoxynucleotide were heightened.
CONCLUSION:Stat3 ASODN participate in apoptosis by enhancing the expression of Bax and reducing the expression of Bcl-2 and C-Myc.
