The effect of short hairpin RNA and DNAzyme gene therapy on latent membrane protein-1 expression in nasopharyngeal carcinoma.
- Author:
Yiqin WANG
1
;
Suling HONG
;
Yucheng YANG
;
Di QIAN
;
Xia KE
Author Information
1. Department of Otolaryngology, Beijing Fengtai Hospital, Beijing,100071, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
DNA, Catalytic;
therapeutic use;
Exons;
Gene Expression Regulation, Viral;
Genetic Therapy;
Humans;
Nasopharyngeal Neoplasms;
genetics;
metabolism;
pathology;
RNA, Small Interfering;
genetics;
therapeutic use;
Transfection;
Viral Matrix Proteins;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(6):270-272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines.
METHOD:The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected.
RESULT:The HNE1 cells expressing green fluorescence in combining group were significantly less than that in RNA interference group (P < 0.01); The inhibition efficiency of green fluorescence in combining group were 86. 31% Wp 88.88% ,which were higher than that in RNA interference group with 75.15%. The detection of LMP1 mRNA and proteins showed that combining group had a higher inhibition ability.
CONCLUSION:Combining gene therapy with pshLMP1 and DNAzyme is superior to pshLMP1 alone to inhibit LMP1 gene expression.
