The study on growth and proliferation of neural stem cells from rats in vitro.
- Author:
Qingguo CHEN
1
;
Yong FU
;
Xianhong WANG
;
Xiaohui WU
;
Rui ZHU
;
Zewen LI
;
Shusheng GONG
Author Information
1. Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Culture Media, Serum-Free;
Embryo, Mammalian;
Female;
Hippocampus;
cytology;
embryology;
Multipotent Stem Cells;
cytology;
Neural Stem Cells;
cytology;
Pregnancy;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(16):747-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.
METHOD:NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.
RESULT:NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.
CONCLUSION:NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
