The expression and significance of NF-kappaB in the rat model of otitis media with effusion experimentally induced by ovalbumin.
- Author:
Shouqin ZHAO
1
;
Hua LIU
;
Demin HAN
;
Yin XIA
;
Erzhong FAN
;
Yang WANG
;
Jie LI
;
Yuanyuan REN
Author Information
1. Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education, Beijing, 100730, China. zhao_sq@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Interleukin-4;
metabolism;
Male;
NF-kappa B;
metabolism;
Otitis Media with Effusion;
chemically induced;
immunology;
metabolism;
Ovalbumin;
adverse effects;
Rats;
Rats, Sprague-Dawley;
Th1 Cells;
immunology;
Th2 Cells;
immunology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(16):751-755
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the T helper cells (Th) predominant differentiation and the modulation of nuclear transcription factors kappa B (NF-kappaB) in middle ear of rat model of otitis media with effusion (OME).
METHOD:Sixteen SD rats were randomly divided into OME (Exp group) and control group (Con group). The expression of NF-kappaB were observed by immunohistochemistry. The level of IFN-gamma and IL-4 in tympanic lavage fluid (TLF) were determined by ELISA.
RESULT:As compared to the Con group , the level of IL-4 and the ratio of Th2/Th1 (IL-4/IFN-gamma) in TLF of Exp group significantly increased (P<0.05), when no significant difference in IFN-gamma levels in TLF was found. The ratio of NF-kappaB p65 positive cells to white cells in temporal bone marrow smears and middle ear mucosa of Exp group was significantly higher than that of Con group (P<0.05). The expression of NF-kappaB p65 in temporal bone marrow smears and middle ear mucosa was signficantly positively correlated with the concentration of IL-4 in TLF of Exp group (P<0.05).
CONCLUSION:The middle ear is capable of mounting an allergic response and subsequent formation of effusion. There is Thl/Th2 immune response imbalance, which polarizes toward Th2 response in the middle ear microenvironment of allergic OME rat model. Moreover , NF-kappaB may participate in regulating Th2 predominant reaction.
