Enhancement of radio sensitivity in nasopharyngeal cancer cells by the own regulation of VEGF expression after adenovirus-E1A gene therapy.
- Author:
Rongrong ZHOU
1
;
Zhiqiang XIAO
;
Yuping LIAO
;
Huaping XIAO
Author Information
1. Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, China. rrzhou99@yahoo.com
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Cell Line, Tumor;
Gene Expression Regulation, Neoplastic;
Genetic Therapy;
Genetic Vectors;
Humans;
Nasopharyngeal Neoplasms;
genetics;
metabolism;
radiotherapy;
Radiation Tolerance;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(20):933-936
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of Ad-E1A gene therapy on the radiosensitivity of nasopharyngeal carcinoma cell by downregulating the expression of VEGF in vitro.
METHOD:The human nasopharyngeal carcinoma CNE-2Z cell lines were investigated. The recombinant adenovirus vector containing E1A gene was used for this study. After CNE-2Z cells was treated with PBS, Ad-beta-gal and Ad-E1A for 48 h, the three groups were irradiated in different doses at 0, 2.4, 6, 8 and 10 Gy, the cytotoxicity was determined by MTT assay and cell cycle was analysis by flow cytometry. The VEGF expression were evaluated by RT-PCR assay and immunocytochemical analysis.
RESULT:Significant cell deaths by IR were observed in a dose dependent manner in the three group CNE-2Z cells. After transduction of the E1A gene into CNE-2Z cells, the sensitivity of these cells to radiation was enhanced than the PBS treated group and Ad-beta-gal treated group. Cell growth inhibition in Ad-E1A group by IR was strongly enhanced than Ad-beta-gal treated group and PBS treated group. RT-PCR assay and immunocytochemical analysis showed VEGF expression was downregulated in Ad-E1A treated group.
CONCLUSION:E1A gene therapy can effectively enhance the nasopharyngeal carcinoma cell sensitivity to the radiotherapy by down-regulating VEGF expression. These findings may pave the way for efficient radiation-gene therapy to NPC in future.
