The relationship between spontaneous apoptosis and radiation-induced apoptosis of laryngeal carcinoma.
- Author:
Weihong XIN
1
;
Ping YAO
;
Defu JIANG
;
Jinsong NI
;
Qianqian CHEN
;
Jun WANG
;
Yingnan KAN
Author Information
1. Department of Otolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130031, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Apoptosis;
radiation effects;
Carcinoma, Squamous Cell;
pathology;
radiotherapy;
Dose-Response Relationship, Radiation;
Female;
Humans;
In Situ Nick-End Labeling;
Laryngeal Neoplasms;
pathology;
radiotherapy;
Male;
Middle Aged
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(21):972-973
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the change of laryngeal carcinoma cell apoptosis before and after radiotherapy.
METHOD:Living tissue of diseased region obtained from 27 cases of laryngeal carcinoma before and during radiotherapy 10, 30 and 60 Gy were used in this study. With terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL), the spontaneous and radiation-induced apoptotic rate (AR)were examined.
RESULT:The AR of living tissue of laryngeal carcinoma before and during radiotherapy 10, 30 and 60 Gy were (21.0 +/- 0.7)%, (60.0 +/- 1.5)%, (42.0 +/- 1.3)%, (25.0 +/- 1.9)%, respectively. The radiation-induced apoptotic rate was significantly higher than that of spontaneous apoptotic rate (P< 0 . 5). In laryngeal carcinoma, spontaneous apoptosis was positively correlated with the radiation-induced apoptosis.
CONCLUSION:Detecting of AR of laryngeal carcinoma cells before and during radiotherapy may be helpful to predict the sensitivity of radiotherapy in laryngeal carcinoma patients.
