Establishment of a cisplatin-induced human nasopharyngeal carcinoma drug-resistant cell line and its biological characteristics.
- Author:
Wen LIN
1
;
Derui LI
;
Yingcheng LIN
;
Caiwen DU
;
Jiongyu CHEN
;
Chaoqun HONG
Author Information
1. Department of Medical Oncology, Affiliated Cancer Hospital, Shantou University Medical College, Shantou, 515031, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Cell Line, Tumor;
Cisplatin;
pharmacology;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
Humans;
Nasopharyngeal Neoplasms
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(21):992-997
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a CDDP-resistant cell line from human nasopharyngeal carcinoma and evaluate its biological characteristics.
METHOD:By continuously exposing and gradually increasing dose of cisplatin (CDDP), a resistant nasopharyngeal carcinoma cell line (HNE1/CDDP) was established. Drug sensitivity of this cell line was detected by MTT assay; the alterations of its biological characteristics were determined using light microscopy, cell counting and flow cytometry (FCM); its ability of adhesion, migration and invasion were also evaluated.
RESULT:HNE1/CDDP cell line was developed after 10 months with stable resistance to cisplatin with the resistance index was 5.83. HNE1/CDDP cell exhibited cross-resistance to many other chemotherapeutic agents (carboplatin, oxaliplatin and etoposide, etc). The morphology of HNE1/CDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while of G0/G1 phase increased compared with parental cells. The ability of adhesion, migration and invasion had no difference between the parental and the resistant cells.
CONCLUSION:HNE1/CDDP cell line shows the typical and stable resistant phenotype and can be used as a research model.
