Effect of apoptosis of vesicular stomatitis virus on tumor cells of nasopharyngeal carcinoma in vivo.
- Author:
Yong FENG
1
;
Gang HE
;
Yang WU
;
Yanjun WEN
Author Information
1. Department of Otorhinolaryngology, People's Hospital of Sichuan, Chengdu, 610072, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cell Line, Tumor;
Humans;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Nasopharyngeal Neoplasms;
Neoplasm Transplantation;
Oncolytic Virotherapy;
Vesiculovirus;
Xenograft Model Antitumor Assays
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(21):995-997
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate apoptosis induced by vesicular stomatitis virus (VSV) in HNE-1 cancer cells of human nasopharyngeal carcinoma by models of nude mice-BALB/c in vivo.
METHOD:HNE-1 cells are collected from culture bottle and infected infra right-side post-back epithelium of nude mice-BALB/c (6 x 10(6) cells/0.1 ml/each mice) to create HNE-1 tumor models of nude mice-BALB/c. When the diameter of HNE-1 tumors is 5 to 8 millimeter, HNE-1 tumor models are treated with VSV (1 x 10(8) pfu /ml) or Saline. By Hoechst 33258-staining under fluorescence microscope, induction of apoptosis by VSV in HNE-1 tumor models are recorded and studied, compared with that by Saline in HNE-1 tumor models in vivo.
RESULT:Compared with control group of saline, apoptosis of HNE-1 cancer cells of human nasopharyngeal carcinoma increase apparently in the remaining tumor cells of nude mice treated by VSV (P < 0.01).
CONCLUSION:The present study suggests that the treatment with VSV could augment the apoptosis cells of human nasopharyngeal carcinoma in vivo.
