A simple method of separating outer hair cells in Guinea pig.
- Author:
Xixun KANG
1
;
Weijia KONG
;
Wanrong LI
;
Xiaorong ZENG
;
Yan YANG
;
Song ZHANG
;
Chankkai GUO
Author Information
1. Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Separation;
methods;
Cochlea;
cytology;
Guinea Pigs;
Hair Cells, Auditory;
cytology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(18):829-831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.
METHOD:The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.
RESULT:A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.
CONCLUSION:If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
